Scientists develop novel method to explore plant-microbe interactions

Argonne scientists awarded funding for bioimaging project to study communication between plants and microbial symbionts.

Humans benefit greatly from our relationship with plants. We rely on them for food, clean air, medicine, fuel and various other products. But plants need other partners to thrive. Many plants grow better by establishing relationships with soil microbes. These microbes help plants acquire nutrients such as nitrogen, phosphorous and iron. In exchange, the plants provide sugars and amino acids to the microbes.

Scientists at the U.S. Department of Energy’s (DOE) Argonne National Laboratory were recently awarded funding from DOE’s Office of Science, Biological and Environmental Research (BER) bioimaging program to understand plant-microbe communication and how it facilitates plant growth and health.

“We can use the wonderful capabilities of the Advanced Photon Source to look at the interface between the microbe and the plant in nanometer resolution.” — Gyorgy Babnigg, bioinformatician/molecular biologist, Argonne

“We want to see what plants are providing to the microbes and what microbes are providing to plants,” said Argonne’s Gyorgy Babnigg, bioinformatician/molecular biologist and lead principal investigator on the project. ​“We want to uncover the signals that affect this symbiotic relationship.”

On a broader scale, understanding plant-microbe interactions can provide insights into improving plant growth and enhancing the products and resources on which people depend. In a world that’s moving toward renewable energy sources, developing products from plants that can replace plastics and other fossil-fuel-derived products becomes even more important.

Over the three-year project, Babnigg and his colleagues at Argonne, the University of Chicago and DOE’s Joint Genome Institute plan to develop a new imaging technique by using engineered plant-growth-promoting bacteria as biosensors. The modified microbes will be able to indicate which resources are being shared between bacteria in the rhizosphere — the region around the plant’s root system — and the plants themselves.

“Bacterial cells have the ability to sense and respond to nutrients, such as amino acids, sugars, small organic acids, as well as signaling molecules in the substances secreted by plant roots,” described Babnigg. Once the cells sense nutrients, they tell the bacteria to use them for energy and growth. The researchers aim to track how much of each nutrient there is around the root system by adding proteins that light up under a microscope to the microbes. Part of the novelty of this color-changing method is that scientists can measure low- and high-abundance compounds in the ground without having to sacrifice the plants.

“We can send these biosensors to other scientists who can then put the microbes into the environments they’re studying to measure the dynamics of compounds in the rhizosphere,” said Babnigg.

Along with developing and testing these biosensors, the scientists will collect microscopic images to further understand the relationship between plants and microbes. ​“We can use the wonderful capabilities of the Advanced Photon Source to look at the interface between the microbe and the plant in nanometer resolution,” Babnigg noted. The team also plans to use these images to assess how components are distributed in the rhizosphere during plant-microbe interactions and how this distribution might change over time and in different environments.

To accelerate discoveries from the images, the researchers will partially automate the imaging process. They plan to develop artificial intelligence (AI)-guided tools for image acquisition and analysis in collaboration with Argonne’s team working on autonomous discovery and the Argonne Leadership Computing Facility. ​“An AI-based, automated system might be able to come up with novel ideas or experimental approaches that we scientists would not think of,” said Babnigg.

The Advanced Photon Source and Argonne Leadership Computing Facility are DOE Office of Science user facilities.

The Argonne Leadership Computing Facility provides supercomputing capabilities to the scientific and engineering community to advance fundamental discovery and understanding in a broad range of disciplines. Supported by the U.S. Department of Energy’s (DOE’s) Office of Science, Advanced Scientific Computing Research (ASCR) program, the ALCF is one of two DOE Leadership Computing Facilities in the nation dedicated to open science.

About the Advanced Photon Source

The U. S. Department of Energy Office of Science’s Advanced Photon Source (APS) at Argonne National Laboratory is one of the world’s most productive X-ray light source facilities. The APS provides high-brightness X-ray beams to a diverse community of researchers in materials science, chemistry, condensed matter physics, the life and environmental sciences, and applied research. These X-rays are ideally suited for explorations of materials and biological structures; elemental distribution; chemical, magnetic, electronic states; and a wide range of technologically important engineering systems from batteries to fuel injector sprays, all of which are the foundations of our nation’s economic, technological, and physical well-being. Each year, more than 5,000 researchers use the APS to produce over 2,000 publications detailing impactful discoveries, and solve more vital biological protein structures than users of any other X-ray light source research facility. APS scientists and engineers innovate technology that is at the heart of advancing accelerator and light-source operations. This includes the insertion devices that produce extreme-brightness X-rays prized by researchers, lenses that focus the X-rays down to a few nanometers, instrumentation that maximizes the way the X-rays interact with samples being studied, and software that gathers and manages the massive quantity of data resulting from discovery research at the APS.

This research used resources of the Advanced Photon Source, a U.S. DOE Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357.

Argonne National Laboratory seeks solutions to pressing national problems in science and technology. The nation’s first national laboratory, Argonne conducts leading-edge basic and applied scientific research in virtually every scientific discipline. Argonne researchers work closely with researchers from hundreds of companies, universities, and federal, state and municipal agencies to help them solve their specific problems, advance America’s scientific leadership and prepare the nation for a better future. With employees from more than 60 nations, Argonne is managed by UChicago Argonne, LLC for the U.S. Department of Energy’s Office of Science.

The U.S. Department of Energy’s Office of Science is the single largest supporter of basic research in the physical sciences in the United States and is working to address some of the most pressing challenges of our time. For more information, visit https://​ener​gy​.gov/​s​c​ience.