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UrduSince the coronavirus emerged in Wuhan, China last month, this pneumonia-like illness has spread at an alarming rate. Just yesterday, the World Health Organization officially declared the outbreak a public health emergency, and as of today, the virus has infected nearly 10,000 people in China, with the death toll soaring to more than 200. More cases continue to appear around the globe, with six coronavirus cases already confirmed in the U.S. In order to contain this pandemic, healthcare workers need to quickly and accurately identify new coronavirus cases so that patients get crucial medical care and transmission can be halted. However, the Chinese labs that can test for coronavirus are currently overwhelmed. There are reports of hospitals in Wuhan having to deny testing for severely ill patients, who are then also denied full-time admission because beds need to be saved for those with confirmed diagnoses. Partly as a result of these testing difficulties, researchers estimate that only 5.1% of coronavirus cases in Wuhan have actually been caught.
A team of researchers led by Leo L.M. Poon, DPhil, of the University of Hong Kong has developed two rapid tests for the coronavirus that could break this diagnostic bottleneck. Using a technology known as real-time reverse transcription polymerase chain reaction (RT-PCR), the tests detect two gene regions that are only found in the Wuhan coronavirus (officially known as 2019-novel-coronavirus) and in other closely related coronaviruses such as SARS. The two gene regions detected by the tests are known as ORF1b and N. Significantly, both tests also take only about 1 hour and 15 minutes to run. This fast turnaround time could enable Chinese labs to greatly increase patient access to coronavirus testing.
To evaluate the performance of these tests, Poon’s team first confirmed that the tests accurately identify genetic material extracted from cells infected with the SARS coronavirus. The researchers also showed that the tests return negative results for samples containing genetic material from other respiratory viruses, demonstrating that the tests accurately differentiate coronavirus infection from other causes of pneumonia. Lastly, Poon’s team used the tests to analyze sputum and throat swab samples from two patients infected with the 2019-novel-coronavirus. The tests correctly gave positive results for both patients.
“Signs of [coronavirus] infection are highly non-specific and these include respiratory symptoms, fever, cough, [shortness of breath], and viral pneumonia,” said Poon. “Thus, diagnostic tests specific for this infection are urgently needed for confirming suspected cases, screening patients, and conducting virus surveillance. The established assays [in this study] can achieve a rapid detection of 2019-novel-coronavirus in human samples, thereby allowing early identification of patients.”
About AACC
Dedicated to achieving better health through laboratory medicine, AACC brings together more than 50,000 clinical laboratory professionals, physicians, research scientists, and business leaders from around the world focused on clinical chemistry, molecular diagnostics, mass spectrometry, translational medicine, lab management, and other areas of progressing laboratory science. Since 1948, AACC has worked to advance the common interests of the field, providing programs that advance scientific collaboration, knowledge, expertise, and innovation. For more information, visit www.aacc.org.
Clinical Chemistry (clinchem.org) is the leading international journal of laboratory medicine, featuring nearly 400 peer-reviewed studies every year that help patients get accurate diagnoses and essential care. This vital research is advancing areas of healthcare ranging from genetic testing and drug monitoring to pediatrics and appropriate test utilization.
Original post https://alertarticles.info
