The goal of the project is to create a membrane-based downstream purification platform for large-scale continuous biomanufacturing of viral vectors and virus-like particles (VLPs).
Viral vectors are tools commonly used by molecular biologists to deliver genetic material into cells, while VLPs are self-assembled viral protein complexes that mimic the native virus structure without being infectious.
“Besides their tremendous impact on human health, the economic impact of large molecule therapeutics is transforming the landscape of the biotechnology industry,” said Bhattacharyya. “For instance, there are a growing number of clinical trials using viral vectors for the delivery of gene therapies.”
Specific objectives of the project include:
- Feedstock production of two common viral vectors for gene therapy applications and VLPs for vaccine applications.
- High-capacity affinity membrane design, fabrication and characterization.
- Downstream membrane chromatography for separating full and empty viral capsids.
- Module design, process optimization and process intensification.
- Employing bioanalytical methods for detection and quantification.
- Providing research opportunities for graduate and undergraduate students.
The development of membrane-based downstream purification platforms for continuous biomanufacturing of viral vectors and VLPs will overcome the technical and economic barriers at multiple fronts and involve close collaborations among researchers at the three different institutions.
“Each collaborating institution offers unique facilities, expertise and resources that will rapidly catalyze the technology development,” said Bhattacharyya. “This is a wonderful example of collaborations between three universities, as well as immense faculty collaborations between engineering, biochemistry, biology and medicine departments.”
Bhattacharyya will serve as the UK principal investigator (PI), while UK faculty members Barbara Knutson, Steve Rankin, Malgorzata (Gosia) Chwatko and Rebecca Dutch, all from the UK Center of Membrane Sciences, will contribute as Co-PIs. The overall PI of the grant is Xianghong Qian, a professor at the University of Arkansas Department of Biomedical Engineering.
Research reported in this publication was supported by the National Science Foundation under Award Number 2218054. The opinions, findings, and conclusions or recommendations expressed are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.