Researchers at the University of North Carolina at Chapel Hill worked with 38 adult male and female rats (18 alcohol-exposed and 20 non-exposed control animals) to explore these changes in brain chemistry and related behavioral deficits following adolescent alcohol exposure. The rats were trained to dig through two types of media, which had different odors, to find a food reward. Subsequently, researchers reversed the test, pairing the reward with a different odor (reversal 1). The test was reversed again, linking the reward back to the original odor (reversal 2). After behavioral testing, samples were taken from relevant brain regions to assess for PV+ neurons and PNNs. The researchers used statistical analysis to explore alcohol-induced changes in the number and size of PV+ neurons and associated PNNs in several brain regions and whether these—and biological sex—were linked to deficits in solving the reward tasks.
While learning the initial test, alcohol-exposed rats made fewer errors than control rats. During reversal 1, however, alcohol-exposed females made more errors than alcohol-exposed males and control females, suggesting difficulty in letting go of an outmoded strategy. During reversal 2, the alcohol-exposed rats struggled overall, performing worse than controls—with female alcohol-exposed rats making more errors than males. The most striking brain differences were in the AIC. There, alcohol-exposed rats had smaller PV+ cells than control rats. The alcohol-exposed rats also had more PV+ neurons surrounded by PNNs and a greater number of PNNs, suggesting that alcohol exposure leads to PNN changes within the AIC. It’s possible that the AICs of the alcohol-exposed rats, especially the females, were less able to detect stimuli relevant during the attention-shifting tasks. Additional statistical analysis showed that early alcohol exposure influenced the increase in the number of PNNs and that the number of PNNs drove errors in reversal 2.
The behavioral and brain findings add to evidence of the link between adolescent alcohol use, neuronal changes, and diminished behavioral flexibility in adult rats. They also reveal that changes within the AIC are likely partly responsible. The authors identify the number of PNNs and the proportion of PV+ neurons surrounded by PNNs in the AIC as valuable targets for future studies on alcohol use, learning, and flexibility.
Effects of adolescent intermittent ethanol exposure on cortical perineuronal net and parvalbumin expression in adulthood significantly mediate behavioral inflexibility. E. D. K. Sullivan, C. A. Dannenhoffer, E. B. Sutherland, E. M. Vidrascu, A. Gomez, C. A. Boettiger, D. L. Robinson. (pp xxx)
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