In a notable advancement, scientists have developed an efficient gene functional analysis method for peach seedlings, overcoming longstanding hurdles in genetic transformation. Utilizing a TRV-based vector system, this innovative approach induces high-frequency gene silencing across diverse plant tissues, enabling in-depth analysis of genes essential for growth and development. The streamlined process notably shortens the transformation and analysis timeline to just 1.5 months, accelerating advancements in horticultural research.