Under optimal conditions, MBP-Ca achieved an impressive calcium chelating rate of 86.26%. Unlike its precursor, MBP-Ca is rich in glutamic acid (32.74%) and aspartic acid (15.10%), signifying its distinct composition.
The study elucidates the mechanism behind calcium binding to MBP, involving carboxyl oxygen, carbonyl oxygen, and amino nitrogen atoms. This interaction leads to intra- and intermolecular interactions, resulting in the folding and aggregation of MBP. Consequently, the secondary structure of MBP-Ca exhibits a 1.90% increase in β-sheet content, a 124.42 nm size expansion, and a transformation from a dense and smooth surface structure to fragmented and coarse blocks.
In comparison to conventional calcium supplements like CaCl2, MBP-Ca demonstrates superior calcium release rates under diverse conditions, including varying temperatures, pH levels, and simulated gastrointestinal digestion. This enhanced release rate is pivotal for calcium transport and absorption, making MBP-Ca a promising dietary calcium supplement with improved bioavailability.
Highlight: A novel calcium supplement with high bioavailability was prepared from protein from mung bean processing wastewater
This groundbreaking research leverages mung bean-derived MBP-Ca to address environmental concerns while providing a practical dietary solution. Further investigations into MBP-Ca’s absorption efficiency and specific mechanisms hold the promise of advancing dietary calcium supplementation and enhancing overall human health.
Funding:This work was supported by the Scientific and Technological Plan Project of Guizhou Province (QKHJC-ZK [2021]177), Discipline and Master’s Site Construction Project of Guiyang University by Guiyang City Financial Support Guiyang University (2021-xk15), and Undergraduate Innovation and Entrepreneurship Training Program of Guiyang University (0203008002045).
Original Url: https://doi.org/10.3390/foods12051058