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UrduThe study represents an important step in understanding the molecular mechanisms underlying idiopathic autism and highlights the significance of short genetic sequences in the regulation of critical cellular functions. The findings offer a new direction for the development of therapies that could improve the quality of life for many individuals and families affected by autism.
This study is based on previous work published in 2018 that identified CPEB4 as a key protein in the regulation of neuronal proteins related to autism. Back in 2018, the researchers observed that, in individuals with autism, the CPEB4 protein lacked a specific neuronal microexon — a tiny segment of genetic material crucial for protein function in the neurons. The work published today in the journal Nature reveals that this small fragment is key for neuronal activity because it preserves the flexibility of CPEB4 to assemble into condensates and disassemble them.
“This study provides new insights into how small modifications in proteins that regulate gene expression can have a significant impact on neuronal development, opening new avenues to explore future therapies,” explains Dr. Méndez, ICREA researcher and head of the Translational Control of Cell Cycle and Differentiation laboratory at IRB Barcelona.
Molecular condensates and gene regulation
The region of the CPEB4 protein that holds the segment lacks a well-defined three-dimensional structure. Proteins with disordered regions can form condensates, which are like small droplets within the cell where molecules, such as messenger RNAs (mRNAs) that code for other proteins involved in neuronal function, are stored in a silenced state. These condensates can assemble and disassemble in response to cellular signals, enabling dynamic regulation of gene expression.
“In this study, we’ve discovered that this neuronal microexon is crucial for maintaining the stability and dynamics of the condensates formed by CPEB4 in neurons. Without the microexon, the condensates are less dynamic and can form solid aggregates that don’t work correctly,” says Dr. Salvatella, ICREA researcher and head of the Laboratory of Molecular Biophysics at IRB Barcelona.
This lack of dynamism prevents the mRNAs stored in these condensates from being released when neurons are stimulated, leading to a decrease in the production of proteins essential for neuronal development and function. Among these mRNA molecules are many of the genes that have previously been linked to autism.
Implications for neuronal development
Proper regulation of these genes is essential during brain development. If these CPEB4 condensates do not function correctly due to the absence of the neuronal microexon, it can lead to disruptions of neuronal development, which are manifested as symptoms of autism. The described mechanism also helps to explain the complexity and heterogeneous nature of idiopathic autism, as this spectrum includes multiple manifestations and varying degrees of severity.
“Our results suggest that even small decreases in the percentage of microexon inclusion can have significant effects. This would explain why some individuals without a gene mutation develop idiopathic autism,” explain Drs. Carla Garcia-Cabau and Anna Bartomeu, IRB Barcelona researchers and first authors of the study.
The concept proposed in this study of gene regulation in neurons through the formation of condensates may also have implications for ageing. Over time, these condensates lose their plasticity, meaning their capacity to assemble and disassemble, which could impair proper neuronal function and promote the development of neurodegenerative diseases.
Possible avenues for future therapies
One of the promising findings of the study is that microexon 4 appears to work “in trans”, which means that it might be possible to introduce this small sequence of amino acids into cells to partially restore CPEB4 function and potentially reverse the symptoms.
“Although we’re still in exploratory stages, this discovery is promising and points to a potential therapeutic approach that could restore CPEB4 function,” says Dr. Méndez. The researchers emphasise that this finding still requires extensive experimental testing, such as studies in animal models and overcoming multiple technical barriers.
Interdisciplinary collaboration and future research
This study is a notable example of how interdisciplinary collaboration can lead to significant advancements in the understanding of complex diseases. By combining approaches from biochemistry, cell biology, biophysics, and neuroscience, the team at IRB Barcelona has managed to unravel a mechanism that could have profound implications for idiopathic autism.
“It’s an achievement that reflects the strength of working in an environment that fosters interaction between different disciplines,” concludes Dr. Salvatella. “We’ll continue to explore this mechanism and its implications, in the hope that we can eventually turn these findings into benefits for individuals affected by autism.”
This work has been made possible through the collaboration of several prestigious institutions and scientists. Among them, special mention is given to Dr. José Lucas, from the Centro de Biología Molecular Severo Ochoa (CBM Severo Ochoa) of CSIC/UAM in Madrid, and Dr. Ruben Hervás, from the Li Ka Shing Faculty of Medicine at the University of Hong Kong. In addition, the research involved groups at the Linderstrøm-Lang Centre for Protein Science of the University of Copenhagen and IBEC. The Centro de Investigación Biomédica en Red del Área de Enfermedades Neurodegenerativas (CIBERNED) of the Instituto de Salud Carlos III, Madrid, University College London, and the University of Barcelona also participated in the work.
This project has been funded mainly by the State Research Agency (AEI) and the European Research Council (ERC).
Related article:
Mis-splicing of a neuronal microexon promotes CPEB4
aggregation in ASD
Carla Garcia Cabau, Anna Bartomeu, Giulio Tesei, Kai Chit Cheung, Julia Pose Utrilla, Sara Picó, Andreea Balaceanu, Berta Duran Arqué, Marcos Fernández Alfara, Judit Martín, Cesare De Pace, Lorena Ruiz Pérez, Jesús García, Giuseppe Battaglia, José J Lucas, Rubén Hervás, Kresten Lindorff Larsen, Raúl Méndez & Xavier Salvatella
Nature (2024) DOI: 10.1038/s41586-024-08289-w
About IRB Barcelona
The Institute for Research in Biomedicine (IRB Barcelona) pursues a society free of disease. To this end, it conducts multidisciplinary research of excellence to cure cancer and other diseases linked to ageing. It establishes technology transfer agreements with the pharmaceutical industry and major hospitals to bring research results closer to society, and organises a range of science outreach activities to engage the public in an open dialogue. IRB Barcelona is an international centre that hosts 400 researchers and more than 30 nationalities. Recognised as a Severo Ochoa Centre of Excellence since 2011, IRB Barcelona is a CERCA centre and member of the Barcelona Institute of Science and Technology (BIST).
