A Breakthrough in Tiny Tool Tuning: Making Microscopic Measurements More Accurate

Fluidic force microscopy (FluidFM) combines the sensitivity of atomic force microscopy with microfluidics’ capabilities, necessitating precise calibration of its cantilevers for reliable data. Traditional methods, however, struggle with the unique internal structure of FluidFM cantilevers, leading to inaccuracies.

A recent study (https://doi.org/10.1038/s41378-023-00629-6) published on February 18, 2024, in the journal Microsystems & Nanoengineering, researchers unveiled an innovative calibration technique for FluidFM micropipette cantilevers, pivotal for exact force measurements in microfluidic environments.

The FluidFM is a tiny tool used in microscopic environments to measure forces with high precision. Unlike traditional methods that often fall short due to the complex inner structure of FluidFM cantilevers, this new approach leverages the cantilever’s resonance frequencies in both air and liquid environments. By focusing on these frequencies, the method circumvents the common pitfalls of the widely-used Sader method, which can introduce errors due to its reliance on geometric and fluidic assumptions that don’t hold up well for FluidFM’s unique cantilever designs. This innovative calibration technique was meticulously tested and validated on data obtained by the HUN-REN Nanobiosensorics Lab, Cytosurge, Nanosurf and Bruker, showing that it not only provides more accurate measurements but also simplifies the calibration process by reducing the effects of noise and eliminating the need for intricate experimental setups.

Dr. Attila Bonyár, the study’s lead author, emphasizes, “Our method simplifies the calibration process, significantly reducing the influence of noise and eliminating the need for complex measurements, marking a significant step forward in the practical application of FluidFM technologies.”

The new calibration method promises enhanced accuracy in force measurements, with profound implications for biological, biophysical, and materials science research. It enables the precise manipulation of cells and nanoparticles, opening new avenues for investigation in these fields.

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References

DOI

10.1038/s41378-023-00629-6

Original Source URL

https://doi.org/10.1038/s41378-023-00629-6

Funding information

This work was supported by the “Lendület” (HAS) research program, the National Research, Development and Innovation Office of Hungary (VEKOP, ELKH topic-fund, “Élvonal” KKP_19 KKP 129936 and KH grants, TKP2021-EGA-04 program financed from the NRDI Fund). The research performed at the Budapest University of Technology and Economics was funded by the National Research, Development, and Innovation Fund of Hungary under Grant TKP2021-EGA-02. We would like to express our thanks to Dr. Torsten Müller from Bruker Nano GmbH for measuring the properties of a micropipette cantilever with their JPK NanoWizard instrument. We would like to express our thanks to Dr. Zoltán Hajnal and Norbert Pap for their help in constructing the 3D model of the cantilever and to Dr. Dario Ossola from Cytosurge AG for his helpful suggestions in formulating the manuscript. A. Bonyár is also grateful for the support of the Hungarian Academy of Engineering and the “MICHELBERGER MESTERDÍJ” Scholarship.

About Microsystems & Nanoengineering

Microsystems & Nanoengineering is an online-only, open access international journal devoted to publishing original research results and reviews on all aspects of Micro and Nano Electro Mechanical Systems from fundamental to applied research. The journal is published by Springer Nature in partnership with the Aerospace Information Research Institute, Chinese Academy of Sciences, supported by the State Key Laboratory of Transducer Technology.