A multi-institutional team of scientists from University of North Carolina, Chapel Hill, University of Massachusetts Chan Medical, and the Environmental Molecular Sciences Laboratory, a Department of Energy Office of Science user facility, led an experimental study to determine the relationships between the subpopulations of cells within these biofilms using Bacillus subtilis, a soil dwelling and biofilm-forming bacterium. The study revealed that current cellular heterogeneity models oversimplify the ability of bacterial cells to differentiate into specific cell types and perform tasks. Data from this research provides a framework with which to further study and make predictions about the diverse cellular phenotypes in B. subtilis biofilms.