The makeup of our microbiomes — the unique communities of bacteria, viruses and other microbes that live in and on us — have been linked, with varying degrees of evidence, to everything from inflammatory bowel disease to athletic performance.
But exactly how could such tiny organisms have such immense influences on a person?
University of California San Diego researchers have created the first-ever map of all the molecules in every organ of a mouse and the ways in which they are modified by microbes. In one surprising example, they discovered that microbes control the structure of bile acids in both mice and people.
The study, published February 26, 2020 in Nature, was led by Pieter Dorrestein, PhD, professor and director of the Collaborative Mass Spectrometry Innovation Center in the Skaggs School of Pharmacy and Pharmaceutical Sciences at UC San Diego, and Robert Quinn, PhD, assistant professor at Michigan State University.
When you change the structure of molecules, such as bile acids, you could change how cells talk to one another and which genes are turned “on” or “off” at a given time, Dorrestein said. And that might have huge consequences for body function and the development of disease.
“We hear a lot about how our own human genes influence our health and behaviors, so it may come as a shock to think that we could have molecules in the body that look and act the way they do not because of our genes, but because of another living organism,” Dorrestein said.
Mapping molecules and microbes in mice
The team compared germ-free (sterile) mice and mice with normal microbes. They used a laboratory technique called mass spectrometry to characterize the non-living molecules in every mouse organ. They identified as many molecules as possible by comparing them to reference structures in the GNPS database, a crowdsourced mass spectrometry repository developed by Dorrestein and collaborators. They also determined which living microbes co-locate with these molecules by sequencing a specific genetic region that acts as a barcode for bacterial types.
In total, they analyzed 768 samples from 96 sites of 29 different organs from four germ-free mice and four mice with normal microbes. The result was a map of all of the molecules found throughout the body of a normal mouse with microbes, and a map of molecules throughout a mouse without microbes.
A comparison of the maps revealed that as much as 70 percent of a mouse’s gut chemistry is determined by its gut microbiome. Even in distant organs, such as the uterus or the brain, approximately 20 percent of molecules were different in the mice with gut microbes.
Bacteria modify bile acids
After constructing these maps, the researchers homed in on one particular family of molecules that appeared to be significantly different when microbes were present: bile acids. Bile acids are primarily produced by the mouse or human liver, and they help digest fats and oils. They can also carry messages throughout the body.
The team discovered bile acids with previously unknown structures in mice with normal microbiomes, but not in germ-free mice. It’s long been known that host liver enzymes add amino acids to bile acids, specifically the amino acids glycine and taurine. But in mice with normal microbiomes, the team found that bacteria are tagging bile acids with other amino acids — phenylalanine, tyrosine and leucine.
“More than 42,000 research papers have been published about bile acids over the course of 170 years,” Quinn said. “And yet these modifications had been overlooked.”
Influence on human health
Curious if the same types of microbe-modified bile acids are found in humans, the researchers used a tool they created, the Mass Spectrometry Search Tool (MASST), to search 1,004 public datasets of samples analyzed with mass spectrometry. They also analyzed by mass spectrometry approximately 3,000 fecal samples submitted to the American Gut Project, a large citizen science effort based at UC San Diego School of Medicine.
Here’s what they found: The unique microbial-modified bile acids the researchers saw in mice were also present in up to 25.3 percent of all human samples in the datasets. These novel bile acids were more abundant in infants and patients with inflammatory bowel disease or cystic fibrosis.
One way bile acids can deliver messages from the gut to other parts of the body is through specific gut receptors called farnesoid X receptors. Bile acids bind and activate the receptors, which then inhibit genes responsible for making more bile acids. The receptors also help regulate liver triglyceride levels and fluid regulation in the intestines, making them important in liver disease and possibly obesity. Several drugs are currently being developed to treat liver disease by activating farnesoid X receptors.
Sure enough, in mice and human cells grown in the lab, Dorrestein, Quinn and team found that the newly discovered, microbe-modified bile acids strongly stimulate farnesoid X receptors, reducing expression of genes responsible for bile acid production in the liver.
The study raises many questions about the role microbes might play in driving liver and other diseases, and in influencing the activity of therapeutics, such as drugs that target farnesoid X receptors.
“This study provides a clear example of how microbes can influence the expression of human genes,” Dorrestein said. “What we still don’t know is the downstream consequences this could have, or how we might be able to intervene to improve human health.”
Additional study co-authors include: Alexey V. Melnik, Alison Vrbanac, Kathryn A. Patras, Mitchell Christy, Zsolt Bodai, Pedro Belda-Ferre, Anupriya Tripathi, Lawton K. Chung, Melissa Quinn, Greg Humphrey, Morgan Panitchpakdi, Kelly Weldon, Alexander Aksenov, Ricardo da Silva, Robert Bussell, Andrew T. Nelson, Mingxun Wang, Eric Leszczynski, Fernando Vargas, Julia M. Gauglitz, Michael J. Meehan, Emily Gentry, Alexis C. Komor, Orit Poulsen, Brigid S. Boland, John T. Chang, William J. Sandborn, Meerana Lim, Kyung E. Rhee, David Ferguson, Manuela Raffatellu, Gabriel G. Haddad, Dionicio Siegel, Victor Nizet, Rob Knight, UC San Diego; Ting Fu, Michael Downes, Ryan D. Welch, Salk Institute for Biological Studies; Julian Avila-Pacheco, Clary Clish, Ramnik J. Xavier, Hera Vlamakis, Broad Institute of MIT and Harvard; Sena Bae, Harvard University; Himel Mallick, Eric A. Franzosa, Jason Lloyd-Price, Curtis Huttenhower, Broad Institute and Harvard University; Taren Thron, Sarkis K. Mazmanian, California Institute of Technology; Timothy D. Arthur, UC San Diego and Broad Institute; Neha Garg, Georgia Institute of Technology and Emory-Children’s Cystic Fibrosis Center; Julie C. Lumeng, University of Michigan; Barbara I. Kazmierczak, Ruchi Jain, Marie Egan, Yale School of Medicine; and Ronald M. Evans, Howard Hughes Medical Institute, Salk Institute.
This research was funded, in part, by the National Institutes of Health (5U01AI124316-03, 1R03CA211211-01, 1R01HL116235 U54DE023798, R24DK110499, GMS10RR029121, R01HD084163, 1KL2TR001444, T15LM011271, DK057978, HL105278, HL088093, ES010337, DK057978, HL105278, HL088093, CA014195, P42ES010337), American Heart Association (grant 13EIA14660045), Howard Hughes Medical Institute, March of Dimes Chair in Molecular and Developmental Biology at the Salk Institute, Samuel Waxman Cancer Research Foundation, Hewitt Medical Foundation, UC San Diego Center for Microbiome Innovation, National Health and Medical Research Council of Australia Project grants (grant 1087297), Leona M. and Harry B. Helmsley Charitable Trust (grant 2017PG-MED001), SWCRF Investigator Award, Ipsen/Biomeasure, Salk Alumni Fellowship, Crohn’s & Colitis Foundation Visiting IBD Research Fellowship, Stand Up to Cancer-Cancer Research UK-Lustgarten Foundation Pancreatic Cancer Dream Team (grant SU2C-AACR-DT-20-16).
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Disclosure: Victor Nizet is founder of, a scientific advisor and has equity in Staurus Pharma, LLC. Curtis Huttenhower is on the scientific advisory board of Seres Therapeutics. Alexander Aksenov and Mingxun Wang are consultants for Ometa Labs LLC. Pieter Dorrestein and Mingxun Wang are consultants for Serenes Therapeutics. William J. Sandborn is a consultant for Abbvie, Allergan, Amgen, Arena Pharmaceuticals, Avexegen Therapeutics, BeiGene, Boehringer Ingelheim, Celgene, Celltrion, Conatus, Cosmo, Escalier Biosciences, Ferring, Forbion, Genentech, Gilead Sciences, Gossamer Bio, Incyte, Janssen, Kyowa Kirin Pharmaceutical Research, Landos Biopharma, Lilly, Oppilan Pharma, Otsuka, Pfizer, Progenity, Prometheus Biosciences (merger of Precision IBD and Prometheus Laboratories), Reistone, Ritter Pharmaceuticals, Robarts Clinical Trials (owned by Health Academic Research Trust, HART), Series Therapeutics, Shire, Sienna Biopharmaceuticals, Sigmoid Biotechnologies, Sterna Biologicals, Sublimity Therapeutics, Takeda, Theravance Biopharma,Tigenix, Tillotts Pharma, UCB Pharma, Ventyx Biosciences, Vimalan Biosciences and Vivelix Pharmaceuticals. Sandborn also owns stock or stock options from BeiGene, Escalier Biosciences, Gossamer Bio, Oppilan Pharma, Prometheus Biosciences (merger of Precision IBD and Prometheus Laboratories), Progenity, Ritter Pharmaceuticals, Ventyx Biosciences and Vimalan Biosciences.</em.
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