A research team has identified two highly efficient glycosyltransferases, AmGT28 and AmGT44, from Astragalus membranaceus, which convert medicarpin to medicarpin 3-O-glucoside and show a preference for pterocarpans over isoflavonoids. These enzymes enable the biosynthesis of glycosides and have been employed in a whole-cell biocatalytic system with high conversion rates. This discovery offers valuable catalytic tools for the production of biologically active pterocarpan glycosides, which have potential applications in pharmaceuticals and biotechnology.
Astragalus root, derived from Astragalus membranaceus, is widely utilized in herbal medicine and dietary supplements due to its rich content of bioactive pterocarpan glycosides. Glycosylation, mediated by UDP-glycosyltransferases (GTs), is essential in the biosynthesis of these compounds. While flavonoid GTs have been well-studied, specific GTs for pterocarpans remain poorly understood , with existing ones showing low selectivity and conversion rates.
A study (DOI: 10.48130/mpb-0024-0013) published in Medicinal Plant Biology on 05 June 2024, aims to address this gap by identifying two efficient pterocarpan GTs, AmGT28 and AmGT44, and establishing a whole-cell catalytic system for pterocarpan glycosylation.
The research team conducted molecular cloning and functional characterization of two glycosyltransferases, AmGT28 and AmGT44, from A.membranaceus. The purified recombinant proteins of AmGT28 and AmGT44 showed an almost 100% conversion rate in catalyzing medicarpin to medicarpin 3-O-glucoside, as confirmed via LC/MS analysis. Furthermore, to assess substrate promiscuity, various aglycones from Astragalus root and derivatives were tested. Both enzymes exhibited high conversion rates for pterocarpans and isoflavonoids, with a new product 7-O-glucoside 5a being identified. AmGT44 exhibited superior conversion rates for specific substrates compared to AmGT28, which was influenced by substrate binding modes. Sugar donor preference was also evaluated, showing that AmGT44 could use UDP-Glc, UDP-Xyl, and UDP-GlcNAc, whereas AmGT28 exhibited limited versatility. Moreover, the optimal reaction conditions for AmGT44 were established, revealing a preference for pterocarpans and a whole-cell biocatalytic system was developed to simplify enzyme usage, thereby achieving high conversion rates for pterocarpans, especially with AmGT44, which reached up to 100% conversion and 78.66 μg/mL titer in scaled-up reactions. However, lower conversion rates for some polar compounds indicated the need for further optimization.
According to the study’s lead researcher, Xue Qiao, “This study has provided efficient catalytic tools for the biosynthesis of pterocarpan glycosides.”
In summary, the study identified two glycosyltransferases, AmGT28 and AmGT44, from A.membranaceus, which were found to efficiently catalyze the conversion of medicarpin to medicarpin 3-O-glucoside, with a preference for pterocarpans over isoflavonoids. A whole-cell biocatalytic system was established, achieving up to 100% conversion rates and high titers. The findings of this research offer valuable tools for biosynthesizing bioactive pterocarpan glycosides, with potential applications in pharmaceuticals and biotechnology. Future work could optimize and expand these systems for industrial-scale production.
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References
DOI
Original Source URL
https://doi.org/10.48130/mpb-0024-0013
Funding information
This work was supported by National Natural Science Foundation of China (82122073, 81973448), National Key Research and Development Program of China (No. 2023YFA0914100), and Key project at central government level: The ability establishment of sustainable use for valuable Chinese medicine resources (2060302).
About Medicinal Plant Biology
Medicinal Plant Biology (e-ISSN 2835-6969) is an open access, online-only, rigorously peer-reviewed academic journal devoted to publishing original research articles, reviews, opinions, methods, editorials, letters, and perspectives on the studies related to all medicinal plants. Subjects include but are not limited to genomic, transcriptomic, proteomic, metabolomic, biochemical, molecular, and omics-based techniques to investigate medicinal properties of plants.
