Starting small to answer the big questions about photosynthesis

New scientific techniques are revealing the intricate role that proteins play in photosynthesis.

Despite being discovered almost 300 years ago, photosynthesis still holds many unanswered questions for science, particularly the way that proteins organise themselves to convert sunlight into chemical energy and at the same time, protect plants from too much sunlight.

Now a collaboration between researchers at the University of Leeds and Kobe University in Japan is developing a novel approach to the investigation of photosynthesis.

Using hybrid membranes that mimic natural plant membranes and advanced microscopes, they are opening photosynthesis to nanoscale investigation – the study of life at less than one billionth of a metre – to reveal the behaviour of individual protein molecules.

Dr Peter Adams, Associate Professor in the School of Physics and Astronomy at the University of Leeds, who supervised the research, said: “For many decades scientists have been developing an understanding of photosynthesis in terms of the biology of whole plants. This research is tackling it at the molecular level and the way proteins interact.

“A greater understanding of photosynthesis will benefit humankind. It will help scientists identify new ways to protect and boost crop yields, as well as inspire technologists to develop new solar-powered materials and components.”

The findings are published in the academic journal

Small

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Photosynthesis happens when photons or packets of light energy cause pigments inside light-harvesting proteins to become excited. The way that these proteins arrange themselves determines how the energy is transferred to other molecules.

It is a complex system that plays out across many different pigments, proteins, and layers of light-harvesting membranes within the plant. Together, it regulates energy absorption, transfer, and the conversion of this energy into other useful forms.

To understand this intricate process, scientists have been using a technique called atomic force microscopy, which is a device capable of revealing components of a membrane that are a few nanometres in size.

The difficulty is that natural plant membranes are very fragile and can be damaged by atomic force microscopy.

But last year, the researchers at Kobe University announced that they had developed a hybrid membrane made up of natural plant material and synthetic lipids that would act as a substitute for a natural plant membrane – and crucially, is more stable when placed in an atomic force microscope.

The team at the University of Leeds used the hybrid membrane and subjected it to atomic force microscopy and another advanced visualisation technique called fluorescence lifetime imaging microscopy, or FLIM.

PhD researcher Sophie Meredith, also from the School of Physics at the University of Leeds, is the lead author in the paper. She said: “The combination of FLIM and atomic force microscopy allowed us to observe the elements of photosynthesis. It gave us an insight into the dynamic behaviors and interactions that take place.

“What is important is that we can control some of the parameters in the hybrid membrane, so we can isolate and control factors, and that helps with experimental investigation.

“In essence, we now have a ‘testbed’ and a suite of advanced imaging tools that will reveal the sub-molecular working of photosynthesis.”

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The research was supported by the Royal Society, the Biotechnology and Biological Sciences Research Council, the Engineering and Physical Sciences Research Council, the Medical Research Council and the Japan Society for the Promotion of Science.

The paper – Model Lipid Membranes Assembled from Natural Plant Thylakoids into 2D Microarray Patterns as a Platform to Assess the Organization and Photophysics of Light-Harvesting Proteins – is available to download at

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For further information, please contact David Lewis in the press office at the University of Leeds:

[email protected]

or on +44 (0)7710 013287

The is an image that goes with the story. The caption is: Light is absorbed by light-harvesting proteins in the plant membrane. The energy excites the proteins and the way they arrange themselves determines the way energy is transferred through the plant.

Please credit the image: Sophie Meredith, University of Leeds.

University of Leeds

The University of Leeds is one of the largest higher education institutions in the UK, with more than 37,000 students from more than 150 different countries, and a member of the Russell Group of research-intensive universities. The University plays a significant role in the Turing, Rosalind Franklin and Royce Institutes.

We are a top ten university for research and impact power in the UK, according to the 2014 Research Excellence Framework, and are in the top 100 of the QS World University Rankings 2019.

The University was awarded a Gold rating by the Government’s Teaching Excellence Framework in 2017, recognising its ‘consistently outstanding’ teaching and learning provision. Twenty-six of our academics have been awarded National Teaching Fellowships – more than any other institution in England, Northern Ireland and Wales – reflecting the excellence of our teaching.

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uk