Researchers perform thousands of mutations to understand amyotrophic lateral sclerosis

• Researchers from
  
  CRG
  
  and
  
  IBEC
  
  in Barcelona use a technique called high-throughput mutagenesis to study Amyotrophic Lateral Sclerosis (ALS), with unexpected results.
• Results showed that aggregation of TDP-43 is not harmful but actually protects cells, changing our understanding of ALS and opening the door to radically new therapeutic approaches.

Amyotrophic lateral sclerosis (ALS) is a devastating and incurable nervous system disease that affects nerve cells in the brain and spinal cord, causing loss of muscle control and normally death within a few years of diagnosis. In ALS, like in other neurodegenerative diseases, specific protein aggregates have long been recogniSed as the pathological hallmarks, but it is not clear whether they represent the actual cause of the disease. Indeed, alleviating aggregation has repeatedly failed as a therapeutic strategy when trying to treat neurodegenerative diseases such as Alzheimer’s disease.

In order to cast more light on this issue, researchers at the

Center of Genomic Regulation (CRG)

and the

Institute of Bioengineering of Catalonia (IBEC)

have applied a novel approach called deep mutagenesis, with unexpected results. “By studying all possible mutations in a protein, we have a much more reliable way to understand toxicity and we are excited to move on to many more proteins implicated in neurodegenerative diseases”, says

Benedetta Bolognesi

, IBEC researcher, CRG Alumni, and first author of the paper.

In a collaboration between the labs of

ICREA

research professors

Ben Lehner

and

Gian Tartaglia

, Benedetta Bolognesi and Andre J. Faure focused on TDP-43, a protein that aggregates in the motor neurons of nearly all ALS patients. They made over 50,000 mutants of TDP-43 and tracked their toxicity to yeast cells. Researchers found that mutant forms that aggregated were actually less toxic than other versions of the protein which instead were forming unusual liquid species in the cells. “This is the exact opposite of what we expected,” Lehner says, and challenges a lot of the assumptions in this field.

It still remains to be established whether aggregation of TDP-43 is also protective in mammalian cells and neurons, something Bolognesi is working on, but if this proves to be the case, it means we will have to entirely change the way we therapeutically address ALS if we want to have significant effects.

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