July 24, 2019 | SCIENION US, Inc. Booth #1579
The main challenges associated with cell line development are maintaining high cell viability and ensuring monoclonality of isolated cells. Unlike flow cell sorters, which induce high stress and compromise cell viabilities, automated cloning using cellenONE is a gentle and precise method to achieve unparalled clonal recoveries in both standard (96 MTP) and high density (1536 MTP) substrates. This is demonstrated with CHO cells, one of the most common cell line used in recombinant therapeutic proteins manufacturing.
The number of wells containing single CHO colonies were counted for each plate (six 96 MTP and one 1536 MTP) and the percent of wells containing single colonies was calculated (Figure 3).
Outstanding clonal recoveries were observed with 96% and 94% of wells containing single colonies in 96 and 1536 MTP, respectively (Figure 2). Additionally, the remaining wells were empty and did not contain multiple colonies. To our knowledge, this is the first time such high clonal recoveries have been demonstrated with CHO cells in both standard (96 MTP) and very high density (1536 MTP) substrates.
This experiment demonstrates the cellenONE as an ideal platform for undertaking automated cloning in both standard and high density substrates as it ensures monoclonality while maintaining outstanding clonal recovery.
Compatibility with high density substrates offers a dramatic increase in throughput to automatically process the equivalent of 16 individual 96 MTP in under an hour leaving personnel free to perform less routine tasks.
In addition to saving time, one can save on consumables and expensive reagents while ensuring documentation and monoclonality of all isolated cells.https://www.cellenion.com/fileadmin/user_upload/Downloadable_Area_pdfs/Application_Note_cellenONE_Unparallel_clonal_recovery_achieved_in_96_and_1536_MTP.pdf